Unknown

Dataset Information

0

Radiation dose effect of DNA repair-related gene expression in mouse white blood cells.


ABSTRACT:

Background

The aim of this study was to screen molecular biomarkers for biodosimetry from DNA repair-related gene expression profiles.

Material/methods

Mice were subjected to whole-body exposure with 60Co gamma rays with a dose range of 0-8 Gy at a dose rate of 0.80 Gy/min. RNA was extracted from the peripheral blood of irradiated mice at 4, 8, 12, 24 and 48hrs post-irradiation. The mRNA transcriptional changes of 11 genes related to DNA damage and repair were detected using real-time quantitative polymerase chain reaction (RT-PCR).

Results

Of the 11 genes examined, CDKN1A (cyclin-dependent kinase inhibitor 1A or p21, Cip1) and ATM (ataxia telangiectasia mutated) expression levels were found to be heavily up- and down-regulated, respectively, with exposure dose increasing at different post-irradiation times. RAD50 (RAD50 homolog), PLK3 (polo-like kinase 3), GADD45A (growth arrest and DNA damage-inducible, alpha), DDB2 (damage-specific DNA-binding protein 2), BBC3 (BCL2-binding component 3) and IER5 (immediate early response 5) gene expression levels were found to undergo significant oscillating changes over a broad dose range of 2-8 Gy at post-exposure time points observed. Three of the genes were found not to change within the observed exposure dose and post-radiation time ranges.

Conclusions

The results of this study add to the biodosimetry with biomarker data pool and will be helpful for constructing appropriate gene expression biomarker systems to evaluate radiation exposure doses.

SUBMITTER: Li MJ 

PROVIDER: S-EPMC3539470 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications

Radiation dose effect of DNA repair-related gene expression in mouse white blood cells.

Li Ming-juan MJ   Wang Wei-wei WW   Chen Shi-wei SW   Shen Qian Q   Min Rui R  

Medical science monitor : international medical journal of experimental and clinical research 20111001 10


<h4>Background</h4>The aim of this study was to screen molecular biomarkers for biodosimetry from DNA repair-related gene expression profiles.<h4>Material/methods</h4>Mice were subjected to whole-body exposure with 60Co gamma rays with a dose range of 0-8 Gy at a dose rate of 0.80 Gy/min. RNA was extracted from the peripheral blood of irradiated mice at 4, 8, 12, 24 and 48hrs post-irradiation. The mRNA transcriptional changes of 11 genes related to DNA damage and repair were detected using real-  ...[more]

Similar Datasets

| S-EPMC11505968 | biostudies-literature
| S-EPMC10585707 | biostudies-literature
| S-EPMC7016656 | biostudies-literature
| S-EPMC8745621 | biostudies-literature
| S-EPMC4526102 | biostudies-literature
| S-EPMC9391341 | biostudies-literature
| S-EPMC10815587 | biostudies-literature
| S-EPMC5412018 | biostudies-literature
| S-EPMC3492462 | biostudies-literature
| S-EPMC4921469 | biostudies-literature