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Osteoblast precursors, but not mature osteoblasts, move into developing and fractured bones along with invading blood vessels.

ABSTRACT: During endochondral bone development, the first osteoblasts differentiate in the perichondrium surrounding avascular cartilaginous rudiments; the source of trabecular osteoblasts inside the later bone is, however, unknown. Here, we generated tamoxifen-inducible transgenic mice bred to Rosa26R-LacZ reporter mice to follow the fates of stage-selective subsets of osteoblast lineage cells. Pulse-chase studies showed that osterix-expressing osteoblast precursors, labeled in the perichondrium prior to vascular invasion of the cartilage, give rise to trabecular osteoblasts, osteocytes, and stromal cells inside the developing bone. Throughout the translocation, some precursors were found to intimately associate with invading blood vessels, in pericyte-like fashion. A similar coinvasion occurs during endochondral healing of bone fractures. In contrast, perichondrial mature osteoblasts did not exhibit perivascular localization and remained in the outer cortex of developing bones. These findings reveal the specific involvement of immature osteoblast precursors in the coupled vascular and osteogenic transformation essential to endochondral bone development and repair.

SUBMITTER: Maes C

PROVIDER: S-EPMC3540406 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature

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Osteoblast precursors, but not mature osteoblasts, move into developing and fractured bones along with invading blood vessels.

Maes Christa C Kobayashi Tatsuya T Selig Martin K MK Torrekens Sophie S Roth Sanford I SI Mackem Susan S Carmeliet Geert G Kronenberg Henry M HM

Developmental cell 20100801 2

During endochondral bone development, the first osteoblasts differentiate in the perichondrium surrounding avascular cartilaginous rudiments; the source of trabecular osteoblasts inside the later bone is, however, unknown. Here, we generated tamoxifen-inducible transgenic mice bred to Rosa26R-LacZ reporter mice to follow the fates of stage-selective subsets of osteoblast lineage cells. Pulse-chase studies showed that osterix-expressing osteoblast precursors, labeled in the perichondrium prior to ...[more]

PMID: 20708594

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Dataset Information

Osteoblast precursors, but not mature osteoblasts, move into developing and fractured bones along with invading blood vessels.

Publications

Osteoblast precursors, but not mature osteoblasts, move into developing and fractured bones along with invading blood vessels.

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