Dataset Information

Analysis of plant gums and saccharide materials in paint samples: comparison of GC-MS analytical procedures and databases.

ABSTRACT: UNLABELLED: BACKGROUND:Saccharide materials have been used for centuries as binding media, to paint, write and illuminate manuscripts and to apply metallic leaf decorations. Although the technical literature often reports on the use of plant gums as binders, actually several other saccharide materials can be encountered in paint samples, not only as major binders, but also as additives. In the literature, there are a variety of analytical procedures that utilize GC-MS to characterize saccharide materials in paint samples, however the chromatographic profiles are often extremely different and it is impossible to compare them and reliably identify the paint binder. RESULTS:This paper presents a comparison between two different analytical procedures based on GC-MS for the analysis of saccharide materials in works-of-art. The research presented here evaluates the influence of the analytical procedure used, and how it impacts the sugar profiles obtained from the analysis of paint samples that contain saccharide materials. The procedures have been developed, optimised and systematically used to characterise plant gums at the Getty Conservation Institute in Los Angeles, USA (GCI) and the Department of Chemistry and Industrial Chemistry of the University of Pisa, Italy (DCCI). The main steps of the analytical procedures and their optimisation are discussed. CONCLUSIONS:The results presented highlight that the two methods give comparable sugar profiles, whether the samples analysed are simple raw materials, pigmented and unpigmented paint replicas, or paint samples collected from hundreds of centuries old polychrome art objects. A common database of sugar profiles of reference materials commonly found in paint samples was thus compiled. The database presents data also from those materials that only contain a minor saccharide fraction. This database highlights how many sources of saccharides can be found in a paint sample, representing an important step forward in the problem of identifying polysaccharide binders in paint samples.

SUBMITTER: Lluveras-Tenorio A

PROVIDER: S-EPMC3541984 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Analysis of plant gums and saccharide materials in paint samples: comparison of GC-MS analytical procedures and databases.

Lluveras-Tenorio Anna A Mazurek Joy J Restivo Annalaura A Colombini Maria Perla MP Bonaduce Ilaria I

Chemistry Central journal 20121010 1

<h4>Unlabelled</h4><h4>Background</h4>Saccharide materials have been used for centuries as binding media, to paint, write and illuminate manuscripts and to apply metallic leaf decorations. Although the technical literature often reports on the use of plant gums as binders, actually several other saccharide materials can be encountered in paint samples, not only as major binders, but also as additives. In the literature, there are a variety of analytical procedures that utilize GC-MS to character ...[more]

PMID: 23050842

Similar Datasets

Project description:Highlights • Malondialdehyde (MDA) is an important biomarker of oxidative stress (lipid peroxidation)• MDA and [1,3-dideutero]MDA (d2-MDA) for use as internal standard react with pentafluorobenzyl bromide (PFB-Br) in aqueous acetone to form di-PFB derivatives.• GC–MS/MS allows for highly accurate measurement of MDA in human plasma, serum and urine.• Many pre-analytical factors including hemolysis may compromise measurement of biological MDA.• Sample storage and study design of long-term clinical studies are important factors to be considered. Malondialdehyde (MDA; 1,3-propanedial, OHC-CH2-CHO) is one of the most frequently measured biomarkers of oxidative stress in plasma and serum. L-Arginine (Arg) is the substrate of nitric oxide synthases (NOS), which convert L-arginine to nitric oxide (NO) and L-citrulline. The Arg/NO pathway comprises several members, including the endogenous NOS-activity inhibitor asymmetric dimethylarginine (ADMA) and its major metabolite dimethyl amine (DMA), and nitrite and nitrate, the major NO metabolites. Reliable measurement of MDA and members of the Arg/NO pathway in plasma, serum, urine and in other biological samples, such as saliva and cerebrospinal fluid, is highly challenging both for analytical and pre-analytical reasons. In our group, we use validated gas chromatography-mass spectrometry (GC–MS) and gas chromatography-tandem mass spectrometry (GC–MS/MS) methods for the quantitative determination in clinical studies of MDA as a biomarker of oxidative stress, and various Arg/NO metabolites that describe the status of this pathway. Here, the importance of pre-analytical issues, which has emerged from the use of GC–MS and GC–MS/MS in clinico-pharmacological studies, is discussed. Paradigmatically, two studies on the long-term oral administration of L-arginine dihydrochloride to patients suffering from peripheral arterial occlusive disease (PAOD) or coronary artery disease (CAD) were considered. Pre-analytical issues that were addressed include blood sampling, plasma or serum storage, study design (notably in long-term studies), and the alternative of measuring MDA in human urine.

Dataset Information

Analysis of plant gums and saccharide materials in paint samples: comparison of GC-MS analytical procedures and databases.

Publications

Analysis of plant gums and saccharide materials in paint samples: comparison of GC-MS analytical procedures and databases.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets