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Transcriptomic markers meet the real world: finding diagnostic signatures of corticosteroid treatment in commercial beef samples.

ABSTRACT: BACKGROUND: The use of growth-promoters in beef cattle, despite the EU ban, remains a frequent practice. The use of transcriptomic markers has already proposed to identify indirect evidence of anabolic hormone treatment. So far, such approach has been tested in experimentally treated animals. Here, for the first time commercial samples were analyzed. RESULTS: Quantitative determination of Dexamethasone (DEX) residues in the urine collected at the slaughterhouse was performed by Liquid Chromatography-Mass Spectrometry (LC-MS). DNA-microarray technology was used to obtain transcriptomic profiles of skeletal muscle in commercial samples and negative controls. LC-MS confirmed the presence of low level of DEX residues in the urine of the commercial samples suspect for histological classification. Principal Component Analysis (PCA) on microarray data identified two clusters of samples. One cluster included negative controls and a subset of commercial samples, while a second cluster included part of the specimens collected at the slaughterhouse together with positives for corticosteroid treatment based on thymus histology and LC-MS. Functional analysis of the differentially expressed genes (3961) between the two groups provided further evidence that animals clustering with positive samples might have been treated with corticosteroids. These suspect samples could be reliably classified with a specific classification tool (Prediction Analysis of Microarray) using just two genes. CONCLUSIONS: Despite broad variation observed in gene expression profiles, the present study showed that DNA-microarrays can be used to find transcriptomic signatures of putative anabolic treatments and that gene expression markers could represent a useful screening tool.

SUBMITTER: Pegolo S

PROVIDER: S-EPMC3541986 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

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Transcriptomic markers meet the real world: finding diagnostic signatures of corticosteroid treatment in commercial beef samples.

Pegolo Sara S Gallina Guglielmo G Montesissa Clara C Capolongo Francesca F Ferraresso Serena S Pellizzari Caterina C Poppi Lisa L Castagnaro Massimo M Bargelloni Luca L

BMC veterinary research 20121030

<h4>Background</h4>The use of growth-promoters in beef cattle, despite the EU ban, remains a frequent practice. The use of transcriptomic markers has already proposed to identify indirect evidence of anabolic hormone treatment. So far, such approach has been tested in experimentally treated animals. Here, for the first time commercial samples were analyzed.<h4>Results</h4>Quantitative determination of Dexamethasone (DEX) residues in the urine collected at the slaughterhouse was performed by Liqu ...[more]

PMID: 23110699

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Transcriptomic markers meet the real world: finding diagnostic signatures of corticosteroid treatment in commercial beef samples.

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Transcriptomic markers meet the real world: finding diagnostic signatures of corticosteroid treatment in commercial beef samples.

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