Deep sequencing of RNA from ancient maize kernels.
Ontology highlight
ABSTRACT:
SUBMITTER: Fordyce SL
PROVIDER: S-EPMC3543400 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Publications
Deep sequencing of RNA from ancient maize kernels.
Fordyce Sarah L SL Ávila-Arcos Maria C MC Rasmussen Morten M Cappellini Enrico E Romero-Navarro J Alberto JA Wales Nathan N Alquezar-Planas David E DE Penfield Steven S Brown Terence A TA Vielle-Calzada Jean-Philippe JP Montiel Rafael R Jørgensen Tina T Odegaard Nancy N Jacobs Michael M Arriaza Bernardo B Higham Thomas F G TF Ramsey Christopher Bronk CB Willerslev Eske E Gilbert M Thomas P MT
PloS one 20130111 1
The characterization of biomolecules from ancient samples can shed otherwise unobtainable insights into the past. Despite the fundamental role of transcriptomal change in evolution, the potential of ancient RNA remains unexploited - perhaps due to dogma associated with the fragility of RNA. We hypothesize that seeds offer a plausible refuge for long-term RNA survival, due to the fundamental role of RNA during seed germination. Using RNA-Seq on cDNA synthesized from nucleic acid extracts, we vali ...[more]
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