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Semitransparent nanostructured films for imaging mass spectrometry and optical microscopy.

ABSTRACT: Semitransparent porous silicon substrates have been developed for pairing nanostructure-initiator mass spectrometry (NIMS) imaging with traditional optical-based microscopy techniques. Substrates were optimized to generate the largest NIMS signal while maintaining sufficient transparency to allow visible light to pass through for optical microscopy. Using these substrates, both phase-contrast and NIMS images of phospholipids from a scratch-wounded cell monolayer were obtained. NIMS images were generated using a spatial resolution of 14 ?m. Coupled with further improvements in spatial resolution, this approach may allow for the localization of intact biological molecules within cells without the need for labeling.

SUBMITTER: Forsythe JG

PROVIDER: S-EPMC3544556 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature

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Semitransparent nanostructured films for imaging mass spectrometry and optical microscopy.

Forsythe Jay G JG Broussard Joshua A JA Lawrie Jenifer L JL Kliman Michal M Jiao Yang Y Weiss Sharon M SM Webb Donna J DJ McLean John A JA

Analytical chemistry 20121127 24

Semitransparent porous silicon substrates have been developed for pairing nanostructure-initiator mass spectrometry (NIMS) imaging with traditional optical-based microscopy techniques. Substrates were optimized to generate the largest NIMS signal while maintaining sufficient transparency to allow visible light to pass through for optical microscopy. Using these substrates, both phase-contrast and NIMS images of phospholipids from a scratch-wounded cell monolayer were obtained. NIMS images were g ...[more]

PMID: 23146026

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