Ontology highlight
ABSTRACT:
SUBMITTER: Bershtein S
PROVIDER: S-EPMC3545112 | biostudies-literature | 2013 Jan
REPOSITORIES: biostudies-literature
Bershtein Shimon S Mu Wanmeng W Serohijos Adrian W R AW Zhou Jingwen J Shakhnovich Eugene I EI
Molecular cell 20121206 1
What are the molecular properties of proteins that fall on the radar of protein quality control (PQC)? Here we mutate the E. coli's gene encoding dihydrofolate reductase (DHFR) and replace it with bacterial orthologous genes to determine how components of PQC modulate fitness effects of these genetic changes. We find that chaperonins GroEL/ES and protease Lon compete for binding to molten globule intermediate of DHFR, resulting in a peculiar symmetry in their action: overexpression of GroEL/ES a ...[more]