Ontology highlight
ABSTRACT:
SUBMITTER: Chen S
PROVIDER: S-EPMC3546169 | biostudies-literature | 2012 Nov
REPOSITORIES: biostudies-literature
Chen Shengxi S Wang Lin L Fahmi Nour Eddine NE Benkovic Stephen J SJ Hecht Sidney M SM
Journal of the American Chemical Society 20121108 46
Because of the lack of sensitivity to small changes in distance by available FRET pairs (a constraint imposed by the dimensions of the enzyme), a DHFR containing two pyrene moieties was prepared to enable the observation of excimer formation. Pyren-1-ylalanine was introduced into DHFR positions 16 and 49 using an in vitro expression system in the presence of pyren-1-ylalanyl-tRNA(CUA). Excimer formation (λ(ex) 342 nm; λ(em) 481 nm) was observed in the modified DHFR, which retained its catalytic ...[more]