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Two pyrenylalanines in dihydrofolate reductase form an excimer enabling the study of protein dynamics.

ABSTRACT: Because of the lack of sensitivity to small changes in distance by available FRET pairs (a constraint imposed by the dimensions of the enzyme), a DHFR containing two pyrene moieties was prepared to enable the observation of excimer formation. Pyren-1-ylalanine was introduced into DHFR positions 16 and 49 using an in vitro expression system in the presence of pyren-1-ylalanyl-tRNA(CUA). Excimer formation (?(ex) 342 nm; ?(em) 481 nm) was observed in the modified DHFR, which retained its catalytic competence and was studied under multiple and single turnover conditions. The excimer appeared to follow a protein conformational change after the H transfer involving the relative position and orientation of the pyrene moieties and is likely associated with product dissociation.

SUBMITTER: Chen S

PROVIDER: S-EPMC3546169 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature

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Two pyrenylalanines in dihydrofolate reductase form an excimer enabling the study of protein dynamics.

Chen Shengxi S Wang Lin L Fahmi Nour Eddine NE Benkovic Stephen J SJ Hecht Sidney M SM

Journal of the American Chemical Society 20121108 46

Because of the lack of sensitivity to small changes in distance by available FRET pairs (a constraint imposed by the dimensions of the enzyme), a DHFR containing two pyrene moieties was prepared to enable the observation of excimer formation. Pyren-1-ylalanine was introduced into DHFR positions 16 and 49 using an in vitro expression system in the presence of pyren-1-ylalanyl-tRNA(CUA). Excimer formation (λ(ex) 342 nm; λ(em) 481 nm) was observed in the modified DHFR, which retained its catalytic ...[more]

PMID: 23116258

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