IL28B polymorphism and cytomegalovirus predict response to treatment in Egyptian HCV type 4 patients.

ABSTRACT:

Aim

To test whether the status of positive cytomegalovirus (CMV) DNA detection adds to the predictive value of IL28B and to further categorize C/T allele carriers.

Methods

This study included 166 chronic hepatitis C (CHC) patients who received combined interferon and ribavirin therapy for 48 wk, 84 spontaneous hepatitis C virus (HCV) resolvers who were positive for IgG anti-HCV antibody and negative for HCV RNA, and 100 healthy subjects who were negative for both HCV antibodies and RNA as controls. Genomic DNA from peripheral blood was used for IL28B rs.12979860 single nucleotide polymorphism (SNP) and CMV DNA detection. A 139 bp fragment containing IL28B SNP was amplified in all subjects by polymerase chain reaction using a specifically designed primer. Then the IL28B rs.12

SUBMITTER: El Awady MK 

PROVIDER: S-EPMC3547570 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature