Mammalian heart renewal by pre-existing cardiomyocytes.
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ABSTRACT:
SUBMITTER: Senyo SE
PROVIDER: S-EPMC3548046 | biostudies-literature | 2013 Jan
REPOSITORIES: biostudies-literature
Publications
Mammalian heart renewal by pre-existing cardiomyocytes.
Senyo Samuel E SE Steinhauser Matthew L ML Pizzimenti Christie L CL Yang Vicky K VK Cai Lei L Wang Mei M Wu Ting-Di TD Guerquin-Kern Jean-Luc JL Lechene Claude P CP Lee Richard T RT
Nature 20121205 7432
Although recent studies have revealed that heart cells are generated in adult mammals, the frequency of generation and the source of new heart cells are not yet known. Some studies suggest a high rate of stem cell activity with differentiation of progenitors to cardiomyocytes. Other studies suggest that new cardiomyocytes are born at a very low rate, and that they may be derived from the division of pre-existing cardiomyocytes. Here we show, by combining two different pulse-chase approaches--gen ...[more]
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Project description:Connexins are a family of membrane-spanning proteins named according to their molecular weight. They are known to form membrane channels mediating cell-cell communication, which play an essential role in the propagation of electrical activity in the heart. Cx26 has been described in a number of tissues but not in the heart, and its mutations are frequently associated with deafness and skin diseases. The aim of this study was to assess the possible Cx26 expression in heart tissues of different mammalian species and to demonstrate its localization at level of cardiomyocytes. Samples of pig, human and rat heart and H9c2 cells were used for our research. Immunohistochemical and molecular biology techniques were employed to test the expression of Cx26. Interestingly, this connexin was found in cardiomyocytes, at level of clusters scattered over the cell cytoplasm but not at level of the intercalated discs where the other cardiac connexins are usually located. Furthermore, the expression of Cx26 in H9c2 myoblast cells increased when they were differentiated into cardiac-like phenotype. To our knowledge, the expression of Cx26 in pig, human and rat has been demonstrated for the first time in the present paper.
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