Design of tandem genes cluster and expression vector for biosynthesis of soybean isoflavones.
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ABSTRACT:
SUBMITTER: Xia XL
PROVIDER: S-EPMC3550378 | biostudies-literature | 2009 Jan
REPOSITORIES: biostudies-literature
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Design of tandem genes cluster and expression vector for biosynthesis of soybean isoflavones.
Xia Xun-Li XL Yang Guang-Xiao GX He Guang-Yuan GY
Physiology and molecular biology of plants : an international journal of functional plant biology 20090101 1
A tandem gene cluster CHS-CHI-IFS (rIFS) for secondary metabolites of plant isoflavones was constructed by using the chalcone synthase (CHS), chalcone isomerase (CHI), and isoflavone synthase (IFS) (GenBank accession numbers EU526827, EU526829, EU526830) in a single recombination event with the pET22b vector. The resulting expression vector pET-rIFS was heterogeneously expressed. The highlights of the vector include ease of handling, high efficiency and universal application among diverse plant ...[more]
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