Dataset Information

Molecular Characterization of a Nucleopolyhedrovirus Newly Isolated from Ophiusa disjungens in China.

ABSTRACT: Ophiusa disjungens nucleopolyhedrovirus (OpdiNPV) was newly found in Guangdong Province, China. Using BamHI, EcoRI, HindIII, PstI, XhoI, XbaI digestion, the size of the OpdiNPV genome was estimated to be 92,000 base pair. The pstI-G genomic fragment of OpdiNPV was cloned and sequenced. Searches of databases identified at least four open reading frames (ORFs) within this fragment. These ORFs encode odv-e66 (EU 623602), p87/vp80 (EU 732665), odv-ec43 (EU617337) and ac108 gene (EU 732666) respectively. The phylogenetic tree of NPVs based on the combined sequences of odv-ec43 and ac108 indicated that OpdiNPV was most closely related to Mamestra configurata NPV-A and Mamestra configurata NPV-B. The characterization of OpdiNPV in this paper would provide better understanding molecular properties of this virus and be helpful in the development of the newly isolated virus as a biopesticide or an engineered pesticide to control more species of insect pests.

SUBMITTER: Lin T

PROVIDER: S-EPMC3550784 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature

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Molecular Characterization of a Nucleopolyhedrovirus Newly Isolated from Ophiusa disjungens in China.

Lin Tong T Liu Li L Chang Runlei R Lang Guojun G Xu Wen W

Indian journal of virology : an official organ of Indian Virological Society 20120918 3

Ophiusa disjungens nucleopolyhedrovirus (OpdiNPV) was newly found in Guangdong Province, China. Using BamHI, EcoRI, HindIII, PstI, XhoI, XbaI digestion, the size of the OpdiNPV genome was estimated to be 92,000 base pair. The pstI-G genomic fragment of OpdiNPV was cloned and sequenced. Searches of databases identified at least four open reading frames (ORFs) within this fragment. These ORFs encode odv-e66 (EU 623602), p87/vp80 (EU 732665), odv-ec43 (EU617337) and ac108 gene (EU 732666) respectiv ...[more]

PMID: 24293829

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Project description:BackgroundBacillus cereus is a foodborne pathogen commonly found in nature and food and can cause food spoilage and health issues. Although the prevalence of B. cereus in foods has been reported worldwide, the extent of contamination in edible fungi, which has become increasingly popular as traditional or functional food, is largely unknown. Here we investigated the prevalence, toxin genes' distribution, antibiotic resistance, and genetic diversity of B. cereus isolated from edible fungi in China.ResultsSix hundred and ninety-nine edible fungi samples were collected across China, with 198 (28.3%) samples found to be contaminated by B. cereus, with an average contamination level of 55.4 most probable number (MPN)/g. Two hundred and forty-seven B. cereus strains were isolated from the contaminated samples. Seven enterotoxin genes and one cereulide synthetase gene were detected. The detection frequencies of all enterotoxin genes were ≥ 80%, whereas the positive rate of the cesB gene in B. cereus was 3%. Most isolates were resistant to penicillins, β-lactam/β-lactamase inhibitor combinations, cephems, and ansamycins, but were susceptible to penems, aminoglycosides, macrolides, ketolide, glycopeptides, quinolones, phenylpropanol, tetracyclines, lincosamides, streptogramins, and nitrofurans. Meanwhile, 99.6% of all isolates displayed multiple antimicrobial resistance to three or more classes of antimicrobials. Using genetic diversity analysis, all isolates were defined in 171 sequence types (STs), of which 83 isolates were assigned to 78 new STs.ConclusionsThis study provides large-scale insight into the prevalence and potential risk of B. cereus in edible fungi in China. Approximately one-third of the samples were contaminated with B. cereus, and almost all isolates showed multiple antimicrobial resistance. Detection frequencies of all seven enterotoxin genes were equal to or more than 80%. These new findings may indicate a need for proper pre-/post-processing of edible fungi to eliminate B. cereus, thereby preventing the potential risk to public health.

Dataset Information

Molecular Characterization of a Nucleopolyhedrovirus Newly Isolated from Ophiusa disjungens in China.

Publications

Molecular Characterization of a Nucleopolyhedrovirus Newly Isolated from Ophiusa disjungens in China.

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