Dataset Information

Antigenic proteins involved in occupational rhinitis and asthma caused by obeche wood (Triplochiton scleroxylon).

ABSTRACT:

Background

Obeche wood dust is a known cause of occupational asthma where an IgE-mediated mechanism has been demonstrated.

Objective

To characterize the allergenic profile of obeche wood dust and evaluate the reactivity of the proteins by in vitro, ex vivo and in vivo assays in carpenters with confirmed rhinitis and/or asthma

Materials and methods

An in-house obeche extract was obtained, and two IgE binding bands were purified (24 and 12 kDa) and sequenced by N-terminal identity. Specific IgE and IgG, basophil activation tests and skin prick tests (SPTs) were performed with whole extract and purified proteins. CCD binding was analyzed by ELISA inhibition studies.

Results

Sixty-two subjects participated: 12 with confirmed occupational asthma/rhinitis (ORA+), 40 asymptomatic exposed (ORA-), and 10 controls. Of the confirmed subjects, 83% had a positive SPT to obeche. There was a 100% recognition by ELISA in symptomatic subjects vs. 30% and 10% in asymptomatic exposed subjects and controls respectively (p<0.05). Two new proteins were purified, a 24 kDa protein identified as a putative thaumatin-like protein and a 12 kDa gamma-expansin. Both showed allergenic activity in vitro, with the putative thaumatin being the most active, with 92% recognition by ELISA and 100% by basophil activation test in ORA+ subjects. Cross-reactivity due to CCD was ruled out in 82% of cases.

Conclusions

Two proteins of obeche wood were identified and were recognized by a high percentage of symptomatic subjects and by a small proportion of asymptomatic exposed subjects. Further studies are required to evaluate cross reactivity with other plant allergens.

SUBMITTER: Aranda A

PROVIDER: S-EPMC3551955 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Publications

Antigenic proteins involved in occupational rhinitis and asthma caused by obeche wood (Triplochiton scleroxylon).

Aranda Ana A Campo Paloma P Palacin Arantxa A Doña Inmaculada I Gomez-Casado Cristina C Galindo Luisa L Díaz-Perales Araceli A Blanca Miguel M

PloS one 20130122 1

<h4>Background</h4>Obeche wood dust is a known cause of occupational asthma where an IgE-mediated mechanism has been demonstrated.<h4>Objective</h4>To characterize the allergenic profile of obeche wood dust and evaluate the reactivity of the proteins by in vitro, ex vivo and in vivo assays in carpenters with confirmed rhinitis and/or asthma<h4>Materials and methods</h4>An in-house obeche extract was obtained, and two IgE binding bands were purified (24 and 12 kDa) and sequenced by N-terminal ide ...[more]

PMID: 23349765

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Project description:During production, thermally modified wood is processed using the same machining operations as unmodified wood. Machining wood is always accompanied with the creation of dust particles. The smaller they become, the more hazardous they are. Employees are exposed to a greater health hazard when machining thermally modified wood because a considerable amount of fine dust is produced under the same processing conditions than in the case of unmodified wood. The International Agency for Research on Cancer (IARC) states that wood dust causes cancer of the nasal cavity and paranasal sinuses and of the nasopharynx. Wood dust is also associated with toxic effects, irritation of the eyes, nose and throat, dermatitis, and respiratory system effects which include decreased lung capacity, chronic obstructive pulmonary disease, asthma and allergic reactions. In our research, granular composition of particles resulting from the process of longitudinal milling of heat-treated oak and spruce wood under variable conditions (i.e., the temperature of modification of 160, 180, 200 and 220 °C and feed rate of 6, 10 and 15 m.min-1) are presented in the paper. Sieve analysis was used to determine the granular composition of particles. An increase in fine particle fraction when the temperature of modification rises was confirmed by the research. This can be due to the lower strength of thermally modified wood. Moreover, a different effect of the temperature modification on granularity due to the tree species was observed. In the case of oak wood, changes occurred at a temperature of 160 °C and in the case of spruce wood, changes occurred at the temperatures of 200 and 220 °C. At the temperatures of modification of 200 and 220 °C, the dust fraction (i.e., that occurred in the mesh sieves, particles with the size ? 0.08 mm) ranged from 2.99% (oak wood, feed rate of 10 m.min-1) to 8.07% (spruce wood, feed rate of 6 m.min-1). Such particles might have a harmful effect on employee health in wood-processing facilities.

Dataset Information

Antigenic proteins involved in occupational rhinitis and asthma caused by obeche wood (Triplochiton scleroxylon).

Background

Objective

Materials and methods

Results

Conclusions

Publications

Antigenic proteins involved in occupational rhinitis and asthma caused by obeche wood (Triplochiton scleroxylon).

Similar Datasets

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