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Structure and dynamics of a primordial catalytic fold generated by in vitro evolution.

ABSTRACT: Engineering functional protein scaffolds capable of carrying out chemical catalysis is a major challenge in enzyme design. Starting from a noncatalytic protein scaffold, we recently generated a new RNA ligase by in vitro directed evolution. This artificial enzyme lost its original fold and adopted an entirely new structure with substantially enhanced conformational dynamics, demonstrating that a primordial fold with suitable flexibility is sufficient to carry out enzymatic function.

SUBMITTER: Chao FA

PROVIDER: S-EPMC3552148 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature

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Structure and dynamics of a primordial catalytic fold generated by in vitro evolution.

Chao Fa-An FA Morelli Aleardo A Haugner John C JC Churchfield Lewis L Hagmann Leonardo N LN Shi Lei L Masterson Larry R LR Sarangi Ritimukta R Veglia Gianluigi G Seelig Burckhard B

Nature chemical biology 20121209 2

Engineering functional protein scaffolds capable of carrying out chemical catalysis is a major challenge in enzyme design. Starting from a noncatalytic protein scaffold, we recently generated a new RNA ligase by in vitro directed evolution. This artificial enzyme lost its original fold and adopted an entirely new structure with substantially enhanced conformational dynamics, demonstrating that a primordial fold with suitable flexibility is sufficient to carry out enzymatic function. ...[more]

PMID: 23222886

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