Cytoplasmic ATP hydrolysis powers transport of lipopolysaccharide across the periplasm in E. coli.
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ABSTRACT:
SUBMITTER: Okuda S
PROVIDER: S-EPMC3552488 | biostudies-literature | 2012 Nov
REPOSITORIES: biostudies-literature
Publications
Cytoplasmic ATP hydrolysis powers transport of lipopolysaccharide across the periplasm in E. coli.
Okuda Suguru S Freinkman Elizaveta E Kahne Daniel D
Science (New York, N.Y.) 20121108 6111
Millions of molecules of lipopolysaccharide (LPS) must be assembled on the Escherichia coli cell surface each time the cell divides. The biogenesis of LPS requires seven essential lipopolysaccharide transport (Lpt) proteins to move LPS from the inner membrane through the periplasm to the cell surface. However, no intermediate transport states have been observed. We developed methods to observe intermediate LPS molecules bound to Lpt proteins in the process of being transported in vivo. Movement ...[more]
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