Dataset Information
Cell type-dependent induction of DNA damage by 1800 MHz radiofrequency electromagnetic fields does not result in significant cellular dysfunctions.
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ABSTRACT: Background
Although IARC clarifies radiofrequency electromagnetic fields (RF-EMF) as possible human carcinogen, the debate on its health impact continues due to the inconsistent results. Genotoxic effect has been considered as a golden standard to determine if an environmental factor is a carcinogen, but the currently available data for RF-EMF remain controversial. As an environmental stimulus, the effect of RF-EMF on cellular DNA may be subtle. Therefore, more sensitive method and systematic research strategy are warranted to evaluate its genotoxicity.Objectives
To determine whether RF-EMF does induce DNA damage and if the effect is cell-type dependent by adopting a more sensitive method ?H2AX foci formation; and to investigate the biological consequences if RF-EMF does increase ?H2AX foci formation.Methods
Six different types of cells were intermittently exposed to GSM 1800 MHz RF-EMF at a specific absorption rate of 3.0 W/kg for 1 h or 24 h, then subjected to immunostaining with anti-?H2AX antibody. The biological consequences in ?H2AX-elevated cell type were further explored with comet and TUNEL assays, flow cytometry, and cell growth assay.Results
Exposure to RF-EMF for 24 h significantly induced ?H2AX foci formation in Chinese hamster lung cells and Human skin fibroblasts (HSFs), but not the other cells. However, RF-EMF-elevated ?H2AX foci formation in HSF cells did not result in detectable DNA fragmentation, sustainable cell cycle arrest, cell proliferation or viability change. RF-EMF exposure slightly but not significantly increased the cellular ROS level.Conclusions
RF-EMF induces DNA damage in a cell type-dependent manner, but the elevated ?H2AX foci formation in HSF cells does not result in significant cellular dysfunctions.
SUBMITTER: Xu S
PROVIDER: S-EPMC3552808 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Publications
Cell type-dependent induction of DNA damage by 1800 MHz radiofrequency electromagnetic fields does not result in significant cellular dysfunctions.
PloS one 20130123 1
<h4>Background</h4>Although IARC clarifies radiofrequency electromagnetic fields (RF-EMF) as possible human carcinogen, the debate on its health impact continues due to the inconsistent results. Genotoxic effect has been considered as a golden standard to determine if an environmental factor is a carcinogen, but the currently available data for RF-EMF remain controversial. As an environmental stimulus, the effect of RF-EMF on cellular DNA may be subtle. Therefore, more sensitive method and syste ...[more]
