Dataset Information
Targeting of liposomes via PSGL1 for enhanced tumor accumulation.
Ontology highlight
ABSTRACT: Purpose
To improve the delivery of liposomes to tumors using P-selectin glycoprotein ligand 1 (PSGL1) mediated binding to selectin molecules, which are upregulated on tumorassociated endothelium.Methods
PSGL1 was orientated and presented on the surface of liposomes to achieve optimal selectin binding using a novel streptavidin-protein G linker molecule. Loading of PSGL1 liposomes with luciferin allowed their binding to e-selectin and activated HUVEC to be quantified in vitro and their stability, pharmacokinetics and tumor accumulation to be tested in vivo using murine models.Results
PSGL1 liposomes showed 5-fold (p?7-fold (p?3-fold enhancement in the level of delivery to tumors (p?ConclusionsThe technologies and strategies described here may contribute to clinical improvements in the selectivity and efficacy of liposomal drug delivery agents.
SUBMITTER: Carlisle R
PROVIDER: S-EPMC3553414 | biostudies-literature | 2013 Feb
REPOSITORIES: biostudies-literature
Publications
Targeting of liposomes via PSGL1 for enhanced tumor accumulation.
Pharmaceutical research 20120920 2
<h4>Purpose</h4>To improve the delivery of liposomes to tumors using P-selectin glycoprotein ligand 1 (PSGL1) mediated binding to selectin molecules, which are upregulated on tumorassociated endothelium.<h4>Methods</h4>PSGL1 was orientated and presented on the surface of liposomes to achieve optimal selectin binding using a novel streptavidin-protein G linker molecule. Loading of PSGL1 liposomes with luciferin allowed their binding to e-selectin and activated HUVEC to be quantified in vitro and ...[more]