Characterization and Functional Assessment of Mouse PPAR?1 Promoter.

ABSTRACT: BACKGROUND:Peroxisome Proliferator Activated Receptor gamma (PPAR?), a member of nuclear receptor superfamily, comprises two isoforms in mouse. These two isoforms are encoded by different mRNAs, which are arisen by alternative promoter usage. There are two promoter regions upstream of PPAR? gene. A 3 kb fragment, containing several transcription factor binding sites, acts as PPAR?1 promoter region. Thus, expression pattern of PPAR?1 isoform is due to the potential transcription factors that could influence its promoter activity. PPAR?, Retinoid X Receptor (RXR) and Vitamin D Receptor (VDR), as nuclear receptors could influence PPAR? gene expression pattern during several differentiation processes. During neural differentiation, PPAR?1 isoform expression reaches to maximal level at neural precursor cell formation. METHODS:A vast computational analysis was carried out to reveal the PPAR?1 promoter region. The putative promoter region was then subcloned upstream of an EGFP reporter gene. Then the functionality of PPAR?1 promoter was assessed in different cell lines. RESULTS:Results indicated that Rosiglitazone increased PPAR?1 promoter regulated EGFP expression of neural precursor cells during Embryoid Body (EB) formation. Furthermore vitamin D reduced PPAR?1 promoter regulated EGFP expression of neural precursor cells during EB formation through binding to its receptor. CONCLUSION:This study suggests that there are potential response elements for PPAR/RXR and VDR/RXR heterodimers in PPAR?1 isoform promoter. Also VDR/RXR heterodimers may decrease PPAR? expression through binding to its promoter.

SUBMITTER: Lachinani L 

PROVIDER: S-EPMC3558222 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature