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Gene-expression profiles and transcriptional regulatory pathways that underlie the identity and diversity of mouse tissue macrophages.

ABSTRACT: We assessed gene expression in tissue macrophages from various mouse organs. The diversity in gene expression among different populations of macrophages was considerable. Only a few hundred mRNA transcripts were selectively expressed by macrophages rather than dendritic cells, and many of these were not present in all macrophages. Nonetheless, well-characterized surface markers, including MerTK and Fc?R1 (CD64), along with a cluster of previously unidentified transcripts, were distinctly and universally associated with mature tissue macrophages. TCEF3, C/EBP-?, Bach1 and CREG-1 were among the transcriptional regulators predicted to regulate these core macrophage-associated genes. The mRNA encoding other transcription factors, such as Gata6, was associated with single macrophage populations. We further identified how these transcripts and the proteins they encode facilitated distinguishing macrophages from dendritic cells.

SUBMITTER: Gautier EL

PROVIDER: S-EPMC3558276 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature

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Gene-expression profiles and transcriptional regulatory pathways that underlie the identity and diversity of mouse tissue macrophages.

Gautier Emmanuel L EL Shay Tal T Miller Jennifer J Greter Melanie M Jakubzick Claudia C Ivanov Stoyan S Helft Julie J Chow Andrew A Elpek Kutlu G KG Gordonov Simon S Mazloom Amin R AR Ma'ayan Avi A Chua Wei-Jen WJ Hansen Ted H TH Turley Shannon J SJ Merad Miriam M Randolph Gwendalyn J GJ

Nature immunology 20120930 11

We assessed gene expression in tissue macrophages from various mouse organs. The diversity in gene expression among different populations of macrophages was considerable. Only a few hundred mRNA transcripts were selectively expressed by macrophages rather than dendritic cells, and many of these were not present in all macrophages. Nonetheless, well-characterized surface markers, including MerTK and FcγR1 (CD64), along with a cluster of previously unidentified transcripts, were distinctly and uni ...[more]

PMID: 23023392

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Gene-expression profiles and transcriptional regulatory pathways that underlie the identity and diversity of mouse tissue macrophages.

Publications

Gene-expression profiles and transcriptional regulatory pathways that underlie the identity and diversity of mouse tissue macrophages.

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