Unknown

Dataset Information

0

Expression and function of Tec, Itk, and Btk in lymphocytes: evidence for a unique role for Tec.


ABSTRACT: The Tec protein tyrosine kinase is the founding member of a family that includes Btk, Itk, Bmx, and Txk. Btk is essential for B-cell receptor signaling, because mutations in Btk are responsible for X-linked agammaglobulinemia (XLA) in humans and X-linked immunodeficiency (xid) in mice, whereas Itk is involved in T-cell receptor signaling. Tec is expressed in both T and B cells, but its role in antigen receptor signaling is not clear. In this study, we show that Tec protein is expressed at substantially lower levels in primary T and B cells relative to Itk and Btk, respectively. However, Tec is up-regulated upon T-cell activation and in Th1 and Th2 cells. In functional experiments that mimic Tec up-regulation, we find that Tec overexpression in lymphocyte cell lines is sufficient to induce phospholipase Cgamma (PLC-gamma) phosphorylation and NFAT (nuclear factor of activated T cells) activation. In contrast, overexpression of Btk, Itk, or Bmx does not induce NFAT activation. Tec-induced NFAT activation requires PLC-gamma, but not the adapters LAT, SLP-76, and BLNK, which are required for Btk and Itk to couple to PLC-gamma. Finally, we show that the unique effector function for Tec correlates with a unique subcellular localization. We hypothesize that Tec functions in activated and effector T lymphocytes to induce the expression of genes regulated by NFAT transcription factors.

SUBMITTER: Tomlinson MG 

PROVIDER: S-EPMC355844 | biostudies-literature | 2004 Mar

REPOSITORIES: biostudies-literature

altmetric image

Publications

Expression and function of Tec, Itk, and Btk in lymphocytes: evidence for a unique role for Tec.

Tomlinson Michael G MG   Kane Lawrence P LP   Su Jennifer J   Kadlecek Theresa A TA   Mollenauer Marianne N MN   Weiss Arthur A  

Molecular and cellular biology 20040301 6


The Tec protein tyrosine kinase is the founding member of a family that includes Btk, Itk, Bmx, and Txk. Btk is essential for B-cell receptor signaling, because mutations in Btk are responsible for X-linked agammaglobulinemia (XLA) in humans and X-linked immunodeficiency (xid) in mice, whereas Itk is involved in T-cell receptor signaling. Tec is expressed in both T and B cells, but its role in antigen receptor signaling is not clear. In this study, we show that Tec protein is expressed at substa  ...[more]

Similar Datasets

| S-EPMC3631358 | biostudies-literature
| S-EPMC10182296 | biostudies-literature
| S-EPMC2722949 | biostudies-literature
| S-EPMC2890196 | biostudies-literature
| S-EPMC3059023 | biostudies-literature
| S-EPMC2785618 | biostudies-literature
| S-EPMC7343623 | biostudies-literature
| S-EPMC3594397 | biostudies-literature
| S-EPMC7361550 | biostudies-literature
| S-EPMC8458560 | biostudies-literature