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APKC?/? and aPKC? contribute to podocyte differentiation and glomerular maturation.


ABSTRACT: Precise positioning of the highly complex interdigitating podocyte foot processes is critical to form the normal glomerular filtration barrier, but the molecular programs driving this process are unknown. The protein atypical protein kinase C (aPKC)--a component of the Par complex, which localizes to tight junctions and interacts with slit diaphragm proteins--may play a role. Here, we found that the combined deletion of the aPKC?/? and aPKC? isoforms in podocytes associated with incorrectly positioned centrosomes and Golgi apparatus and mislocalized molecules of the slit diaphragm. Furthermore, aPKC-deficient podocytes failed to form the normal network of foot processes, leading to defective glomerular maturation with incomplete capillary formation and mesangiolysis. Our results suggest that aPKC isoforms orchestrate the formation of the podocyte processes essential for normal glomerular development and kidney function. Defective aPKC signaling results in a dramatically simplified glomerular architecture, causing severe proteinuria and perinatal death.

SUBMITTER: Hartleben B 

PROVIDER: S-EPMC3559483 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature

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aPKCλ/ι and aPKCζ contribute to podocyte differentiation and glomerular maturation.

Hartleben Björn B   Widmeier Eugen E   Suhm Martina M   Worthmann Kirstin K   Schell Christoph C   Helmstädter Martin M   Wiech Thorsten T   Walz Gerd G   Leitges Michael M   Schiffer Mario M   Huber Tobias B TB  

Journal of the American Society of Nephrology : JASN 20130118 2


Precise positioning of the highly complex interdigitating podocyte foot processes is critical to form the normal glomerular filtration barrier, but the molecular programs driving this process are unknown. The protein atypical protein kinase C (aPKC)--a component of the Par complex, which localizes to tight junctions and interacts with slit diaphragm proteins--may play a role. Here, we found that the combined deletion of the aPKCλ/ι and aPKCζ isoforms in podocytes associated with incorrectly posi  ...[more]

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