The DnaK/DnaJ chaperone machinery of Salmonella enterica serovar Typhimurium is essential for invasion of epithelial cells and survival within macrophages, leading to systemic infection.

ABSTRACT: Salmonella enterica serovar Typhimurium, similar to various facultative intracellular pathogens, has been shown to respond to the hostile conditions inside macrophages of the host organism by inducing stress proteins, such as DnaK. DnaK forms a chaperone machinery with the cochaperones DnaJ and GrpE. To elucidate the role of the DnaK chaperone machinery in the pathogenesis of S. enterica serovar Typhimurium, we first constructed an insertional mutation in the dnaK-dnaJ operon of pathogenic strain chi3306. The DnaK/DnaJ-depleted mutant was temperature sensitive for growth, that is, nonviable above 39 degrees C. We then isolated a spontaneously occurring revertant of the dnaK-dnaJ-disrupted mutant at 39 degrees C and used it for infection of mice. The mutant lost the ability to cause a letha

SUBMITTER: Takaya A 

PROVIDER: S-EPMC356005 | biostudies-literature | 2004 Mar

REPOSITORIES: biostudies-literature