Optimized surface markers for the prospective isolation of high-quality hiPSCs using flow cytometry selection.
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ABSTRACT:
SUBMITTER: Abujarour R
PROVIDER: S-EPMC3560358 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Publications
Optimized surface markers for the prospective isolation of high-quality hiPSCs using flow cytometry selection.
Abujarour Ramzey R Valamehr Bahram B Robinson Megan M Rezner Betsy B Vranceanu Florin F Flynn Peter P
Scientific reports 20130131
hiPSC derivation and selection remains inefficient; with selection of high quality clones dependent on extensive characterization which is not amenable to high-throughput (HTP) approaches. We recently described the use of a cocktail of small molecules to enhance hiPSC survival and stability in single cell culture and the use of flow cytometry cell sorting in the HTP-derivation of hiPSCs. Here we report an enhanced protocol for the isolation of bona fide hiPSCs in FACS-based selection using an op ...[more]
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