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Ultrabright photoactivatable fluorophores created by reductive caging.

ABSTRACT: Sub-diffraction-limit imaging can be achieved by sequential localization of photoactivatable fluorophores, for which the image resolution depends on the number of photons detected per localization. We report a strategy for fluorophore caging that creates photoactivatable probes with high photon yields. Upon photoactivation, these probes can provide 10(4)-10(6) photons per localization and allow imaging of fixed samples with resolutions of several nanometers. This strategy can be applied to many fluorophores across the visible spectrum.

SUBMITTER: Vaughan JC

PROVIDER: S-EPMC3561463 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature

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Ultrabright photoactivatable fluorophores created by reductive caging.

Vaughan Joshua C JC Jia Shu S Zhuang Xiaowei X

Nature methods 20121028 12

Sub-diffraction-limit imaging can be achieved by sequential localization of photoactivatable fluorophores, for which the image resolution depends on the number of photons detected per localization. We report a strategy for fluorophore caging that creates photoactivatable probes with high photon yields. Upon photoactivation, these probes can provide 10(4)-10(6) photons per localization and allow imaging of fixed samples with resolutions of several nanometers. This strategy can be applied to many ...[more]

PMID: 23103881

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