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Kit-On-A-Lid-Assays for accessible self-contained cell assays.

ABSTRACT: Microscale methods for cell-based assays typically rely on macroscopic reagent handling and fluidic loading protocols that are technically challenging and do not scale with the number of assays favorably. Here, we demonstrate a microfluidic platform technology called "Kit-On-A-Lid-Assay" (KOALA), that enables the creation of self-contained microfluidic cell-based assays, integrating all the steps required to perform cell-based assays. The KOALA platform allows the pre-packaging of reagents, cryopreservation of cell suspensions, thawing of cell suspensions, culture of cells, and operation of whole cell-based assays. The operation of the KOALA platform is user-friendly and consists of bringing together a lid containing the microchannels, and a base containing the pre-packaged reagents, thereby causing fluidic exchange in all the channels simultaneously. We demonstrate that the KOALA cell-based assays can be simply operated from start to finish without any external laboratory equipment.

SUBMITTER: Berthier E

PROVIDER: S-EPMC3562598 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature

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Kit-On-A-Lid-Assays for accessible self-contained cell assays.

Berthier Erwin E Guckenberger David J DJ Cavnar Peter P Huttenlocher Anna A Keller Nancy P NP Beebe David J DJ

Lab on a chip 20130201 3

Microscale methods for cell-based assays typically rely on macroscopic reagent handling and fluidic loading protocols that are technically challenging and do not scale with the number of assays favorably. Here, we demonstrate a microfluidic platform technology called "Kit-On-A-Lid-Assay" (KOALA), that enables the creation of self-contained microfluidic cell-based assays, integrating all the steps required to perform cell-based assays. The KOALA platform allows the pre-packaging of reagents, cryo ...[more]

PMID: 23229806

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