2ab assembly: a methodology for automatable, high-throughput assembly of standard biological parts.
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ABSTRACT:
SUBMITTER: Leguia M
PROVIDER: S-EPMC3563576 | biostudies-literature | 2013 Jan
REPOSITORIES: biostudies-literature
Publications
2ab assembly: a methodology for automatable, high-throughput assembly of standard biological parts.
Leguia Mariana M Brophy Jennifer An JA Densmore Douglas D Asante Angel A Anderson J Christopher JC
Journal of biological engineering 20130110 1
There is growing demand for robust DNA assembly strategies to quickly and accurately fabricate genetic circuits for synthetic biology. One application of this technology is reconstitution of multi-gene assemblies. Here, we integrate a new software tool chain with 2ab assembly and show that it is robust enough to generate 528 distinct composite parts with an error-free success rate of 96%. Finally, we discuss our findings in the context of its implications for biosafety and biosecurity. ...[more]
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