Ontology highlight
ABSTRACT:
SUBMITTER: Benabdallah BF
PROVIDER: S-EPMC3564421 | biostudies-literature | 2013 Jan
REPOSITORIES: biostudies-literature
Benabdallah Basma F BF Duval Arnaud A Rousseau Joel J Chapdelaine Pierre P Holmes Michael C MC Haddad Eli E Tremblay Jacques P JP Beauséjour Christian M CM
Molecular therapy. Nucleic acids 20130129
Zinc finger nucleases (ZFN) can facilitate targeted gene addition to the genome while minimizing the risks of insertional mutagenesis. Here, we used a previously characterized ZFN pair targeting the chemokine (C-C motif) receptor 5 (CCR5) locus to introduce, as a proof of concept, the enhanced green fluorescent protein (eGFP) or the microdystrophin genes into human myoblasts. Using integrase-defective lentiviral vectors (IDLVs) and chimeric adenoviral vectors to transiently deliver template DNA ...[more]