Ontology highlight
ABSTRACT:
SUBMITTER: Lee G
PROVIDER: S-EPMC3565423 | biostudies-literature | 2012 Jun
REPOSITORIES: biostudies-literature
Lee Gwangrog G Bratkowski Matthew A MA Ding Fang F Ke Ailong A Ha Taekjip T
Science (New York, N.Y.) 20120601 6089
Rrp44 (Dis3) is a key catalytic subunit of the yeast exosome complex and can processively digest structured RNA one nucleotide at a time in the 3' to 5' direction. Its motor function is powered by the energy released from the hydrolytic nuclease reaction instead of adenosine triphosphate hydrolysis as in conventional helicases. Single-molecule fluorescence analysis revealed that instead of unwinding RNA in single base pair steps, Rrp44 accumulates the energy released by multiple single nucleotid ...[more]