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Autofluorescence as a tool for structural analysis of biofilms formed by nonpigmented rapidly growing mycobacteria.


ABSTRACT: The structure of biofilms formed by seven nonpigmented rapidly growing mycobacteria, including saprophytes and opportunistic species, was analyzed. Analysis included amount of covered surface, thickness, cell viability, and presence of intrinsic autofluorescence at different times using confocal laser scanning microscopy and image analysis. Autofluorescence was detected inside and outside cells of all mycobacteria.

SUBMITTER: Munoz-Egea MC 

PROVIDER: S-EPMC3568543 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature

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Autofluorescence as a tool for structural analysis of biofilms formed by nonpigmented rapidly growing mycobacteria.

Muñoz-Egea María-Carmen MC   García-Pedrazuela María M   Mahillo Ignacio I   García María Jesús MJ   Esteban Jaime J  

Applied and environmental microbiology 20121130 3


The structure of biofilms formed by seven nonpigmented rapidly growing mycobacteria, including saprophytes and opportunistic species, was analyzed. Analysis included amount of covered surface, thickness, cell viability, and presence of intrinsic autofluorescence at different times using confocal laser scanning microscopy and image analysis. Autofluorescence was detected inside and outside cells of all mycobacteria. ...[more]

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