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Temperature- and salinity-decoupled overproduction of hydroxyectoine by Chromohalobacter salexigens.


ABSTRACT: Hydroxyectoine overproduction by the natural producer Chromohalobacter salexigens is presented in this study. Genetically engineered strains were constructed that at low salinity coexpressed, in a vector derived from a native plasmid, the ectoine (ectABC) and hydroxyectoine (ectD) genes under the control of the ectA promoter, in a temperature-independent manner. Hydroxyectoine production was further improved by increasing the copies of ectD and using a C. salexigens genetic background unable to synthesize ectoines.

SUBMITTER: Rodriguez-Moya J 

PROVIDER: S-EPMC3568561 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature

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Temperature- and salinity-decoupled overproduction of hydroxyectoine by Chromohalobacter salexigens.

Rodríguez-Moya Javier J   Argandoña Montserrat M   Iglesias-Guerra Fernando F   Nieto Joaquín J JJ   Vargas Carmen C  

Applied and environmental microbiology 20121116 3


Hydroxyectoine overproduction by the natural producer Chromohalobacter salexigens is presented in this study. Genetically engineered strains were constructed that at low salinity coexpressed, in a vector derived from a native plasmid, the ectoine (ectABC) and hydroxyectoine (ectD) genes under the control of the ectA promoter, in a temperature-independent manner. Hydroxyectoine production was further improved by increasing the copies of ectD and using a C. salexigens genetic background unable to  ...[more]

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