Recruitment and rearrangement of three different genetic determinants into a conjugative plasmid increase copper resistance in Pseudomonas syringae.
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ABSTRACT:
SUBMITTER: Gutierrez-Barranquero JA
PROVIDER: S-EPMC3568574 | biostudies-literature | 2013 Feb
REPOSITORIES: biostudies-literature
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Recruitment and rearrangement of three different genetic determinants into a conjugative plasmid increase copper resistance in Pseudomonas syringae.
Gutiérrez-Barranquero José A JA de Vicente Antonio A Carrión Víctor J VJ Sundin George W GW Cazorla Francisco M FM
Applied and environmental microbiology 20121126 3
We describe the genetic organization of a copper-resistant plasmid containing copG and cusCBA genes in the plant pathogen Pseudomonas syringae. Chromosomal variants of czcCBA and a plasmid variant of cusCBA were present in different P. syringae pathovar strains. Transformation of the copper-sensitive Pseudomonas syringae pv. syringae FF5 strain with copG or cusCBA conferred copper resistance, and quantitative real-time PCR (qRT-PCR) experiments confirmed their induction by copper. ...[more]
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