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Multiplex PCR assay for direct identification of group B streptococcal alpha-protein-like protein genes.


ABSTRACT: We developed a group B streptococcus multiplex PCR assay which allows, by direct analysis of the amplicon size, determination of the surface protein antigen genes of alpha-C protein, epsilon protein, Rib, Alp2, Alp3, and Alp4. The multiplex PCR assay offers a rapid and simple method of subtyping Streptococcus agalactiae based on surface protein genes.

SUBMITTER: Creti R 

PROVIDER: S-EPMC356896 | biostudies-literature | 2004 Mar

REPOSITORIES: biostudies-literature

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Multiplex PCR assay for direct identification of group B streptococcal alpha-protein-like protein genes.

Creti Roberta R   Fabretti Francesca F   Orefici Graziella G   von Hunolstein Christina C  

Journal of clinical microbiology 20040301 3


We developed a group B streptococcus multiplex PCR assay which allows, by direct analysis of the amplicon size, determination of the surface protein antigen genes of alpha-C protein, epsilon protein, Rib, Alp2, Alp3, and Alp4. The multiplex PCR assay offers a rapid and simple method of subtyping Streptococcus agalactiae based on surface protein genes. ...[more]

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