Ontology highlight
ABSTRACT:
SUBMITTER: Wu C
PROVIDER: S-EPMC3568992 | biostudies-literature | 2012 Mar
REPOSITORIES: biostudies-literature
Wu Cheng C Wei Jiajie J Lin Pen-Jen PJ Tu Liwei L Deutsch Carol C Johnson Arthur E AE Sachs Matthew S MS
Journal of molecular biology 20120105 4
The fungal arginine attenuator peptide (AAP) is a regulatory peptide that controls ribosome function. As a nascent peptide within the ribosome exit tunnel, it acts to stall ribosomes in response to arginine (Arg). We used three approaches to probe the molecular basis for stalling. First, PEGylation assays revealed that the AAP did not undergo overall compaction in the tunnel in response to Arg. Second, site-specific photocross-linking showed that Arg altered the conformation of the wild-type AAP ...[more]