Unknown

Dataset Information

0

The complex of cytochrome f and plastocyanin from Nostoc sp. PCC 7119 is highly dynamic.


ABSTRACT: Cytochrome f (Cyt f) and plastocyanin (Pc) form a highly transient complex as part of the photosynthetic redox chain. The complex from Nostoc sp. PCC 7119 was studied by NMR relaxation spectroscopy with the aim of determining the orientation of Pc relative to Cyt f. Chemical-shift-perturbation analysis showed that the presence of spin labels on the surface of Cyt f does not significantly affect the binding of Pc. The paramagnetic relaxation enhancement results are not consistent with a single orientation of Pc, thus indicating that multiple orientations must occur and suggesting that an encounter state represents a large fraction of the complex.

SUBMITTER: Scanu S 

PROVIDER: S-EPMC3569876 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature

altmetric image

Publications

The complex of cytochrome f and plastocyanin from Nostoc sp. PCC 7119 is highly dynamic.

Scanu Sandra S   Förster Johannes J   Finiguerra Michela G MG   Shabestari Maryam Hashemi MH   Huber Martina M   Ubbink Marcellus M  

Chembiochem : a European journal of chemical biology 20120522 9


Cytochrome f (Cyt f) and plastocyanin (Pc) form a highly transient complex as part of the photosynthetic redox chain. The complex from Nostoc sp. PCC 7119 was studied by NMR relaxation spectroscopy with the aim of determining the orientation of Pc relative to Cyt f. Chemical-shift-perturbation analysis showed that the presence of spin labels on the surface of Cyt f does not significantly affect the binding of Pc. The paramagnetic relaxation enhancement results are not consistent with a single or  ...[more]

Similar Datasets

| S-EPMC2665108 | biostudies-literature
| S-EPMC7865156 | biostudies-literature
| S-EPMC6442461 | biostudies-literature
| S-EPMC2546793 | biostudies-literature
| S-EPMC3766884 | biostudies-literature
| S-EPMC2408588 | biostudies-literature
2018-02-19 | E-MTAB-6459 | biostudies-arrayexpress
| S-EPMC1305281 | biostudies-literature
| S-EPMC4574047 | biostudies-literature
| S-EPMC9750909 | biostudies-literature