Kinetic mechanism of phenylalanine hydroxylase: intrinsic binding and rate constants from single-turnover experiments.
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ABSTRACT: Phenylalanine hydroxylase (PheH) catalyzes the key step in the catabolism of dietary phenylalanine, its hydroxylation to tyrosine using tetrahydrobiopterin (BH(4)) and O(2). A complete kinetic mechanism for PheH was determined by global analysis of single-turnover data in the reaction of PheH?117, a truncated form of the enzyme lacking the N-terminal regulatory domain. Formation of the productive PheH?117-BH(4)-phenylalanine complex begins with the rapid binding of BH(4) (K(d) = 65 ?M). Subsequent addition of phenylalanine to the binary complex to form the productive ternary complex (K(d) = 130 ?M) is approximately 10-fold slower. Both substrates can also bind to the free enzyme to form inhibitory binary complexes. O(2) rapidly binds to the productive ternary complex; this is followed by formation of an unidentified intermediate, which can be detected as a decrease in absorbance at 340 nm, with a rate constant of 140 s(-1). Formation of the 4a-hydroxypterin and Fe(IV)O intermediates is 10-fold slower and is followed by the rapid hydroxylation of the amino acid. Product release is the rate-determining step and largely determines k(cat). Similar reactions using 6-methyltetrahydropterin indicate a preference for the physiological pterin during hydroxylation.
SUBMITTER: Roberts KM
PROVIDER: S-EPMC3572726 | biostudies-literature | 2013 Feb
REPOSITORIES: biostudies-literature
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