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Crosslinks in the cell wall of budding yeast control morphogenesis at the mother-bud neck.

ABSTRACT: Previous work has shown that, in cla4? cells of budding yeast, where septin ring organization is compromised, the chitin ring at the mother-daughter neck becomes essential for prevention of neck widening and for cytokinesis. Here, we show that it is not the chitin ring per se, but its linkage to ?(1-3)glucan that is required for control of neck growth. When in a cla4? background, crh1? crh2? mutants, in which the chitin ring is not connected to ?(1-3)glucan, grew very slowly and showed wide and growing necks, elongated buds and swollen cells with large vacuoles. A similar behavior was elicited by inhibition of the Crh proteins. This aberrant morphology matched that of cla4? chs3? cells, which have no chitin at the neck. Thus, this is a clear case in which a specific chemical bond between two substances, chitin and glucan, is essential for the control of morphogenesis. This defines a new paradigm, in which chemistry regulates growth.

SUBMITTER: Blanco N

PROVIDER: S-EPMC3575710 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature

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Crosslinks in the cell wall of budding yeast control morphogenesis at the mother-bud neck.

Blanco Noelia N Reidy Michael M Arroyo Javier J Cabib Enrico E

Journal of cell science 20121017 Pt 23

Previous work has shown that, in cla4Δ cells of budding yeast, where septin ring organization is compromised, the chitin ring at the mother-daughter neck becomes essential for prevention of neck widening and for cytokinesis. Here, we show that it is not the chitin ring per se, but its linkage to β(1-3)glucan that is required for control of neck growth. When in a cla4Δ background, crh1Δ crh2Δ mutants, in which the chitin ring is not connected to β(1-3)glucan, grew very slowly and showed wide and ...[more]

PMID: 23077181

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