Unknown

Dataset Information

0

DNA target sequence identification mechanism for dimer-active protein complexes.


ABSTRACT: Sequence-specific DNA-binding proteins must quickly and reliably localize specific target sites on DNA. This search process has been well characterized for monomeric proteins, but it remains poorly understood for systems that require assembly into dimers or oligomers at the target site. We present a single-molecule study of the target-search mechanism of protelomerase TelK, a recombinase-like protein that is only active as a dimer. We show that TelK undergoes 1D diffusion on non-target DNA as a monomer, and it immobilizes upon dimerization even in the absence of a DNA target site. We further show that dimeric TelK condenses non-target DNA, forming a tightly bound nucleoprotein complex. Together with theoretical calculations and molecular dynamics simulations, we present a novel target-search model for TelK, which may be generalizable to other dimer and oligomer-active proteins.

SUBMITTER: Landry MP 

PROVIDER: S-EPMC3575837 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature

altmetric image

Publications

DNA target sequence identification mechanism for dimer-active protein complexes.

Landry Markita P MP   Zou Xueqing X   Wang Lei L   Huang Wai Mun WM   Schulten Klaus K   Chemla Yann R YR  

Nucleic acids research 20121228 4


Sequence-specific DNA-binding proteins must quickly and reliably localize specific target sites on DNA. This search process has been well characterized for monomeric proteins, but it remains poorly understood for systems that require assembly into dimers or oligomers at the target site. We present a single-molecule study of the target-search mechanism of protelomerase TelK, a recombinase-like protein that is only active as a dimer. We show that TelK undergoes 1D diffusion on non-target DNA as a  ...[more]

Similar Datasets

| S-EPMC3197616 | biostudies-literature
2015-08-01 | E-GEOD-70388 | biostudies-arrayexpress
2015-08-01 | GSE70402 | GEO
2015-08-01 | GSE70388 | GEO
2015-08-01 | E-GEOD-70387 | biostudies-arrayexpress
| S-EPMC4553207 | biostudies-literature
| S-EPMC413530 | biostudies-other
2015-08-01 | GSE70387 | GEO
| S-EPMC2194778 | biostudies-literature
| S-EPMC7810915 | biostudies-literature