Detection of disordered regions in globular proteins using ¹³C-detected NMR.
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ABSTRACT:
SUBMITTER: Gray FL
PROVIDER: S-EPMC3575925 | biostudies-literature | 2012 Dec
REPOSITORIES: biostudies-literature
Publications
Detection of disordered regions in globular proteins using ¹³C-detected NMR.
Gray Felicia L V FL Murai Marcelo J MJ Grembecka Jolanta J Cierpicki Tomasz T
Protein science : a publication of the Protein Society 20121201 12
Characterization of disordered regions in globular proteins constitutes a significant challenge. Here, we report an approach based on ¹³C-detected nuclear magnetic resonance experiments for the identification and assignment of disordered regions in large proteins. Using this method, we demonstrate that disordered fragments can be accurately identified in two homologs of menin, a globular protein with a molecular weight over 50 kDa. Our work provides an efficient way to characterize disordered fr ...[more]
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