Ontology highlight
ABSTRACT:
SUBMITTER: Minh DD
PROVIDER: S-EPMC3576546 | biostudies-literature | 2013 Feb
REPOSITORIES: biostudies-literature
Minh David D L DD Makowski Lee L
Biophysical journal 20130201 4
A new way to use wide-angle x-ray solution scattering to study protein-ligand binding is presented. First, scattering patterns are measured at different protein and ligand concentrations. Multivariate curve resolution based on singular value decomposition and global analysis is applied to estimate the binding affinities and reference patterns (i.e., the scattering patterns of individual components). As validated by simulation, Bayesian confidence intervals provide accurate uncertainty estimates ...[more]