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Dual RMCE for efficient re-engineering of mouse mutant alleles.

ABSTRACT: We have developed dual recombinase-mediated cassette exchange (dRMCE) to efficiently re-engineer the thousands of available conditional alleles in mouse embryonic stem cells. dRMCE takes advantage of the wild-type loxP and FRT sites present in these conditional alleles and in many gene-trap lines. dRMCE is a scalable, flexible tool to introduce tags, reporters and mutant coding regions into an endogenous locus of interest in an easy and highly efficient manner.

SUBMITTER: Osterwalder M

PROVIDER: S-EPMC3576631 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature

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Dual RMCE for efficient re-engineering of mouse mutant alleles.

Osterwalder Marco M Galli Antonella A Rosen Barry B Skarnes William C WC Zeller Rolf R Lopez-Rios Javier J

Nature methods 20101017 11

We have developed dual recombinase-mediated cassette exchange (dRMCE) to efficiently re-engineer the thousands of available conditional alleles in mouse embryonic stem cells. dRMCE takes advantage of the wild-type loxP and FRT sites present in these conditional alleles and in many gene-trap lines. dRMCE is a scalable, flexible tool to introduce tags, reporters and mutant coding regions into an endogenous locus of interest in an easy and highly efficient manner. ...[more]

PMID: 20953177

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