Ontology highlight
ABSTRACT:
SUBMITTER: Ravera E
PROVIDER: S-EPMC3578289 | biostudies-literature | 2013 Feb
REPOSITORIES: biostudies-literature
Journal of the American Chemical Society 20130125 5
Using the 480 kDa iron-storage protein complex, apoferritin (ApoF), as an example, we demonstrate that sizable dynamic nuclear polarization (DNP) enhancements can be obtained on sedimented protein samples. In sedimented solute DNP (SedDNP), the biradical polarizing agent is co-sedimented with the protein, but in the absence of a glass-forming agent. We observe DNP enhancement factors ε > 40 at a magnetic field of 5 T and temperatures below 90 K, indicating that the protein sediment state is "gla ...[more]