Ontology highlight
ABSTRACT:
SUBMITTER: Cheng K
PROVIDER: S-EPMC3578835 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Cheng Keding K Drebot Mike M McCrea Joanne J Peterson Lorea L Lee David D McCorrister Stuart S Nickel Richard R Gerbasi Alyssia A Sloan Angela A Janella Debra D Van Domselaar Gary G Beniac Daniel D Booth Tim T Chui Linda L Tabor Helen H Westmacott Garrett G Gilmour Matthew M Wang Gehua G
PloS one 20130221 2
Serotyping is the long-standing gold standard method to determine E. coli H antigens; however, this method requires a panel of H-antigen specific antibodies and often culture-based induction of the H-antigen flagellar motility. In this study, a rapid and accurate method to isolate and identify the Escherichia coli (E. coli) H flagellar antigen was developed using membrane filtration and liquid chromatography-tandem mass spectrometry (LC-MS/MS). Flagella were isolated from pure culture, digested ...[more]