Ontology highlight
ABSTRACT:
SUBMITTER: Heo WD
PROVIDER: S-EPMC3579512 | biostudies-literature | 2006 Dec
REPOSITORIES: biostudies-literature
Heo Won Do WD Inoue Takanari T Park Wei Sun WS Kim Man Lyang ML Park Byung Ouk BO Wandless Thomas J TJ Meyer Tobias T
Science (New York, N.Y.) 20061109 5804
Many signaling, cytoskeletal, and transport proteins have to be localized to the plasma membrane (PM) in order to carry out their function. We surveyed PM-targeting mechanisms by imaging the subcellular localization of 125 fluorescent protein-conjugated Ras, Rab, Arf, and Rho proteins. Out of 48 proteins that were PM-localized, 37 contained clusters of positively charged amino acids. To test whether these polybasic clusters bind negatively charged phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2 ...[more]