Ontology highlight
ABSTRACT:
SUBMITTER: Abe N
PROVIDER: S-EPMC3579524 | biostudies-literature | 2008 May
REPOSITORIES: biostudies-literature
Journal of cell science 20080414 Pt 9
Endocytosis and recycling of membrane proteins are key processes for nutrient uptake, receptor signaling and synaptic transmission. Different steps in these fission and fusion cycles have been proposed to be regulated by physiological changes in plasma membrane (PM) phosphatidylinositol (4,5)-bisphosphate [PtdIns(4,5)P(2)] concentration. Here, we use a chemical enzyme-translocation strategy to rapidly reduce PM PtdIns(4,5)P(2) levels while monitoring clathrin-mediated endocytosis and recycling. ...[more]