Lessons on conditional gene targeting in mouse adipose tissue.
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ABSTRACT:
SUBMITTER: Lee KY
PROVIDER: S-EPMC3581196 | biostudies-literature | 2013 Mar
REPOSITORIES: biostudies-literature
Publications
Lessons on conditional gene targeting in mouse adipose tissue.
Lee Kevin Y KY Russell Steven J SJ Ussar Siegfried S Boucher Jeremie J Vernochet Cecile C Mori Marcelo A MA Smyth Graham G Rourk Michael M Cederquist Carly C Rosen Evan D ED Kahn Barbara B BB Kahn C Ronald CR
Diabetes 20130115 3
Conditional gene targeting has been extensively used for in vivo analysis of gene function in adipocyte cell biology but often with debate over the tissue specificity and the efficacy of inactivation. To directly compare the specificity and efficacy of different Cre lines in mediating adipocyte specific recombination, transgenic Cre lines driven by the adipocyte protein 2 (aP2) and adiponectin (Adipoq) gene promoters, as well as a tamoxifen-inducible Cre driven by the aP2 gene promoter (iaP2), w ...[more]
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