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A cis-acting element present within the Gag open reading frame negatively impacts on the activity of the HIV-1 IRES.


ABSTRACT: Translation initiation from the human immunodeficiency virus type-1 (HIV-1) mRNA can occur through a cap or an IRES dependent mechanism. Cap-dependent translation initiation of the HIV-1 mRNA can be inhibited by the instability element (INS)-1, a cis-acting regulatory element present within the gag open reading frame (ORF). In this study we evaluated the impact of the INS-1 on HIV-1 IRES-mediated translation initiation. Using heterologous bicistronic mRNAs, we show that the INS-1 negatively impact on HIV-1 IRES-driven translation in in vitro and in cell-based experiments. Additionally, our results show that the inhibitory effect of the INS-1 is not general to all IRESes since it does not hinder translation driven by the HCV IRES. The inhibition by the INS-1 was partially rescued in cells by the overexpression of the viral Rev protein or hnRNPA1.

SUBMITTER: Valiente-Echeverria F 

PROVIDER: S-EPMC3581557 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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A cis-acting element present within the Gag open reading frame negatively impacts on the activity of the HIV-1 IRES.

Valiente-Echeverría Fernando F   Vallejos Maricarmen M   Monette Anne A   Pino Karla K   Letelier Alejandro A   Huidobro-Toro J Pablo JP   Mouland Andrew J AJ   López-Lastra Marcelo M  

PloS one 20130225 2


Translation initiation from the human immunodeficiency virus type-1 (HIV-1) mRNA can occur through a cap or an IRES dependent mechanism. Cap-dependent translation initiation of the HIV-1 mRNA can be inhibited by the instability element (INS)-1, a cis-acting regulatory element present within the gag open reading frame (ORF). In this study we evaluated the impact of the INS-1 on HIV-1 IRES-mediated translation initiation. Using heterologous bicistronic mRNAs, we show that the INS-1 negatively impa  ...[more]

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