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ViralFusionSeq: accurately discover viral integration events and reconstruct fusion transcripts at single-base resolution.


ABSTRACT: SUMMARY: Insertional mutagenesis from virus infection is an important pathogenic risk for the development of cancer. Despite the advent of high-throughput sequencing, discovery of viral integration sites and expressed viral fusion events are still limited. Here, we present ViralFusionSeq (VFS), which combines soft-clipping information, read-pair analysis and targeted de novo assembly to discover and annotate viral-human fusions. VFS was used in an RNA-Seq experiment, simulated DNA-Seq experiment and re-analysis of published DNA-Seq datasets. Our experiments demonstrated that VFS is both sensitive and highly accurate. AVAILABILITY: VFS is distributed under GPL version 3 at http://hkbic.cuhk.edu.hk/software/viralfusionseq

SUBMITTER: Li JW 

PROVIDER: S-EPMC3582262 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature

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ViralFusionSeq: accurately discover viral integration events and reconstruct fusion transcripts at single-base resolution.

Li Jing-Woei JW   Wan Raymond R   Yu Chi-Shing CS   Co Ngai Na NN   Wong Nathalie N   Chan Ting-Fung TF  

Bioinformatics (Oxford, England) 20130112 5


<h4>Summary</h4>Insertional mutagenesis from virus infection is an important pathogenic risk for the development of cancer. Despite the advent of high-throughput sequencing, discovery of viral integration sites and expressed viral fusion events are still limited. Here, we present ViralFusionSeq (VFS), which combines soft-clipping information, read-pair analysis and targeted de novo assembly to discover and annotate viral-human fusions. VFS was used in an RNA-Seq experiment, simulated DNA-Seq exp  ...[more]

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