Ontology highlight
ABSTRACT:
SUBMITTER: Suarez J
PROVIDER: S-EPMC3584414 | biostudies-literature | 2013 Feb
REPOSITORIES: biostudies-literature
Chemistry & biology 20130201 2
Purine nucleoside phosphorylase (PNP) is a target for leukemia, gout, and autoimmune disorders. Dynamic motion of catalytic site loops has been implicated in catalysis, but experimental evidence was lacking. We replaced catalytic site groups His257 or His64 with 6-fluoro-tryptophan (6FW) as site-specific NMR probes. Conformational adjustments in the 6FW-His257-helical and His64-6FW-loop regions were characterized in PNP phosphate-bound enzyme and in complexes with catalytic site ligands, includi ...[more]