Ontology highlight
ABSTRACT:
SUBMITTER: Jalah R
PROVIDER: S-EPMC3585113 | biostudies-literature | 2013 Mar
REPOSITORIES: biostudies-literature
Jalah Rashmi R Rosati Margherita M Ganneru Brunda B Pilkington Guy R GR Valentin Antonio A Kulkarni Viraj V Bergamaschi Cristina C Chowdhury Bhabadeb B Zhang Gen-Mu GM Beach Rachel Kelly RK Alicea Candido C Broderick Kate E KE Sardesai Niranjan Y NY Pavlakis George N GN Felber Barbara K BK
The Journal of biological chemistry 20130107 9
IL-12 is a 70-kDa heterodimeric cytokine composed of the p35 and p40 subunits. To maximize cytokine production from plasmid DNA, molecular steps controlling IL-12p70 biosynthesis at the posttranscriptional and posttranslational levels were investigated. We show that the combination of RNA/codon-optimized gene sequences and fine-tuning of the relative expression levels of the two subunits within a cell resulted in increased production of the IL-12p70 heterodimer. We found that the p40 subunit pla ...[more]