Ontology highlight
ABSTRACT:
SUBMITTER: Lee HW
PROVIDER: S-EPMC3586730 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Lee Hong-Won HW Kyung Taeyoon T Yoo Janghyun J Kim Tackhoon T Chung Chaeuk C Ryu Ji Young JY Lee Hanki H Park Kihyun K Lee Sangkyu S Jones Walton D WD Lim Dae-Sik DS Hyeon Changbong C Heo Won Do WD Yoon Tae-Young TY
Nature communications 20130101
Co-immunoprecipitation (co-IP) has become a standard technique, but its protein-band output provides only static, qualitative information about protein-protein interactions. Here we demonstrate a real-time single-molecule co-IP technique that generates real-time videos of individual protein-protein interactions as they occur in unpurified cell extracts. By analysing single Ras-Raf interactions with a 50-ms time resolution, we have observed transient intermediates of the protein-protein interacti ...[more]